Mass Spectrometry Selectivity, Specifically

Investigation of drift gas selectivity in high resolution ion mobility spectrometry with mass spectrometry detection.

Recent studies in electrospray ionization (ESI)/ion mobility spectrometry (IMS) have focussed on employing different drift gases to alter separation efficiency for some molecules. This study investigates four structurally similar classes of molecules (cocaine and metabolites, amphetamines, benzodiazepines, and small peptides) to determine the effect of structure on relative mobility changes in ...

Mass Spectrometry

Mass Spectrometry

study of Quaternary environmental changes; a review. Quaternary Science Reviews, 1, 245–313. Peterson, L. C., Haug, G. H., Hughen, K. A., and Roehl, U., 2000. Rapid changes in the hydrologic cycle of the tropical Atlantic during the last glacial. Science, 290, 1947–1951. Pike, J., and Kemp, A. E. S., 1996. Preparation and analysis techniques for studies of laminated sediments. In Kemp, A. E. S....

Use of Nanostructure-Initiator Mass Spectrometry to Deduce Selectivity of Reaction in Glycoside Hydrolases

Chemically synthesized nanostructure-initiator mass spectrometry (NIMS) probes derivatized with tetrasaccharides were used to study the reactivity of representative Clostridium thermocellum β-glucosidase, endoglucanases, and cellobiohydrolase. Diagnostic patterns for reactions of these different classes of enzymes were observed. Results show sequential removal of glucose by the β-glucosidase an...

Profiling the selectivity of DNA ligases in an array format with mass spectrometry

This article describes a method for the global profiling of the substrate specificities of DNA ligases and illustrates examples using the Taq and T4 DNA ligases. The method combines oligonucleotide arrays, which offer the benefits of high throughput and multiplexed assays, with mass spectrometry to permit label-free assays of ligase activity. Arrays were prepared by immobilizing ternary biotin-...